Evaluation and characterization of EIA measuring autoantibodies against oxidized LDL …
· The aim of this study was to optimize EIA measuring autoantibodies against copper oxidized LDL and to compare different ways to present the results. Optimized conditions were as follows: native and copper oxidized LDLs used as antigen, coating concentration 2 μg/ml on polysorp plates, 1% HSA as a blocking agent, sample dilution 1:50, HRP-conjugate dilution 1:8000, and 0.2% HSA in sample and ...